A method for effecting a dermatological biopsy procedure and a hand-held instrument for use in such procedure. The instrument has a pen-like housing with an aperture and a biopsy site marker. A syringe for dispensing an anesthetic to a selected marked biopsy site on a patient's skin and a biopsy-sampling blade such as a skin punch or scraper and a wound closure device for closing the biopsy wound with a suture, adhesive or cauterant are contained within the housing. The aperture of the instrument is placed directly against the marked biopsy site and the syringe, biopsy blade and wound closure device are controlled to alternately extend and retract through the aperture and operatively effect anesthetic dispensing, biopsy sampling and wound closure.