A sealed mattress cover assembly (100) is fabricated having a plurality of seal assemblies (110) disposed upon a mattress cover (102). The mattress cover (102) is formed having a top sheet (103) a bottom sheet (104) and a side sheet (105) extending between the two. An opening (closed via a cover closure (106)) is incorporated within the mattress cover (102) allowing the cover to be placed over the mattress. The seal assemblies (110) are formed having an outer frame (112), an inner frame (120) and a seal membrane (114) sandwiched between the two frame members. A seal aperture (116) is formed in the center of the seal membrane (114). Functionality interfacing conduits (108) are placed through the seal aperture (116), allowing the seal membrane (114) to form a seal about the exterior of the conduits (108).